Chromotek gfp-trap® magnetic agarose
WebMay 12, 2024 · How to plan an immunoprecipitation of your GFP-fusion protein when using the ChromoTek GFP-Trap®PreambleThis document provides practical informatio. ... Magnetic agarose beads: Magnetic beads: Matrix: Agarose, 4% highly cross-linked: Agarose, 6% cross-linked: Silica, non-porous: Partical size: 45-165μm: 20-40μm: 0.5-1μm: WebProteintech ChromoTek GFP-Trap™ Agarose GFP-Trap™ Agarose is an affinity resin for IP of GFP-fusion proteins. Brand: Proteintech GTA-20 Additional Details : Weight : 0.00240kg View more versions of this product Product Code. 17393343 378.00 € / 20 reactions Quantity Estimated Shipment: 28-03-2024 Add to basket Description …
Chromotek gfp-trap® magnetic agarose
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WebApr 11, 2024 · Sir2:eGFP was immunoprecipitated with ChromoTek GFP-Trap® Magnetic Agarose (ChromoTek, Planegg, Germany). Elution was performed by boiling the samples in LaemmLi buffer supplemented 200 mM DTT and proteins precipitated by addition of 4 volumes 1:1 methanol:acetone. Protein pellets were resuspended in urea 6 M, 50 mM … WebChromotek GFP-Trap is supposed to be superior beads. So if anyone can think as in how to promote the binding of the complex (GFP-SG1) of +HS samples to GFP-Trap, it would …
WebChromoTek GmbH. F2H®-Kit Basic: Kit comprises F2H®-BHK Cell Line, 1 vial 5 X 106 frozen cells (f2h-bhk); 100 µg Platform Reagent (f2h-p100); and 30 µg Control-DNA (f2h-c30) Platform Reagent: Transfection supplement enabling assembly of the nuclear PPI-platform to anchor GFP-tagged proteins; for 125 transfections (24-well format) or 500 ... WebThe ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to …
WebGFP and Cfap91-GFP proteins were purified from the cytoplasm of GFP or Cfap91-GFP overexpressing cells using magnetic agarose beads-conjugated anti-GFP antibodies and after washings, incubated with a cytoplasmic extract obtained from cells overexpressing HA-Ccdc39 proteins (118 kDa). WebChromoTek pioneered the development and commercialization of alpaca Nanobodies. As a market and product leader for high quality and reliable Nanobody-based reagents, we assist scientists’...
WebThe GFP-Trap® specifically binds to most common GFP derivatives. It is a ready-to-use affinity resin for immunoprecipitation of GFP-fusion proteins. It consists of an anti-GFP Nanobody/ VHH...
WebGFP-Trap® Magnetic Agarose Kit Product code: gtmak-20 Contact [email protected] ChromoTek GmbH Am Klopferspitz 19 82152 Planegg … how did my credit card get hackedWebApr 10, 2024 · Total cell extracts of yeast cells untreated or treated with CHX were incubated in ChromoTek GFP-Trap Agarose beads (Proteintech) for 2.5 h at 4 °C to pull-down AGP2-GFP tagged-associated proteins. After incubation, the beads coupled to proteins were washed with washing buffer (10 mM Tris/Cl pH 7.5, 0.5 mM EDTA, 150 … how did my computer screen flip sidewayshow did my credit report get frozenWebBartlesville Urgent Care. 3. Urgent Care. “I'm wondering what the point of having an urgent care is if it's not open in the evening.” more. 3. Ascension St. John Clinic Urgent Care - … how many sisters of silence are thereWebGFP-Trap™ Agarose is an affinity resin for IP of GFP-fusion proteins. Brand: Proteintech GTA-20. Additional Details : Weight : 0.00240kg. View more versions of this product. … how many sister ships did titanic haveWebChromoTek Spot-Cap® is a premium affinity resin optimized for one-step protein purification. Spot-tagged proteins can be purified with high yields using… how did my debit card get compromisedWebChromotek GFP-Trap is supposed to be superior beads. So if anyone can think as in how to promote the binding of the complex (GFP-SG1) of +HS samples to GFP-Trap, it would be very helpful. how many sisters does zayn have